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Antibacterial activity of an Atmospheric pressure plasma jet on periodontal pathogen+ in vitro models
EAO Online Library. KIM B. Oct 9, 2018; 232616; P-BR-67
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Non‐+thermal plasma discharges are applied to the sterilization of surgical instruments and the decontamination of microorganisms recently. The Atmosphere Pressure Plasma Jet (APPJ), one of the Dielectric Barrier Discharges (DBD), can be a promising tool for the non‐+thermal bacteria sterilization due to high amount of radical generation.The aim of this study was to evaluate the in vitro antibacterial activity of APPJ as an effective approach against periodontal pathogen.To evaluate antibacterial activity, the viability and morphology of Porphyromonas gingivalis (P. gingivalis) were investigated after expose to APPJ. P. gingivalis was inoculated in 96 well multiplate and exposed to APPJ for 10, 30, 60 and 180 seconds, and then the viability and morphology were measured. The bacteria was incubated on Ti disc and in chamber slides for 3 days to form biofilm and exposed to APPJ for 1, 3 and 5 minutes, and then adhesion aspect of the bacteria were investigated with SEM and CLSM.Treatment with APPJ reduced the quantity of P. gingivalis all exposure times. In addition, in TEM images, the most cells showed that the cytoplasmic contents were completely leaked out after expose to APPJ for 5 minutes, leaving two distinct membranes. **In SEM images, little bacteria was detected on Ti disc after treatment for 3 minutes with APPJ. In CLSM images, dead cells were increased over times and little dead cells were investigated after treatment for 5 minutes.The present results demonstrated that APPJ were effective for killing P. gingivalis and removing biofilm on culture dish and Ti disc. The results suggest the potential of APPJ for antimicrobial applications in dentistry. In particular, the disinfection of biofilm-contaminated microstructured Ti implant surface may be a promising option. In this context, APPJ could provide a novel approach used in combination with surgical treatment.**
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