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Evaluation of cells growth on a new plga bmm scaffold.
EAO Online Library. Mizutani F. Oct 9, 2018; 232600; P-BR-52
Fábio Shiniti Mizutani
Fábio Shiniti Mizutani
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Abstract
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Scaffolds in GBR must have some desirable factors- it must allow adhesion and cell growth+ no component or product of its degradation should induce inflammatory or toxicity reactions+ the material has to be manufactured in three-dimensional structure with a porosity that should provide high surface area for cell-scaffold interaction providing space for extracellular matrix deposition+ and it must be a long lasting resorbable material. The aim of this study was to analyze the osteoblast culture cells from calvarial rats in a new poly lactic acid-co-glycolic (PLGA 75-25) scaffolds associated to bovine mineralized matrix (BMM), observing spread, growth and cell scaffold interaction.The isolation of the cells was achieved through sequential enzymatic digestion with trypsin and collagenase solution. The cells were pipetted onto the scaffolds and glass coverslips contained in 24 well plates at the density of 2x10 4 cells well and were grown for periods of up to seven days. **All samples, were fixed with Karnosky's solution, post-fixed with 1% osmium tetroxide in 0.05M cacodylate buffer, pH 7.2, for 1 hour at room temperature in the hood. The samples were washed with distilled water and submitted to baths in increasing solutions of ethanol. They were dehydrated using the critical-point drying and subjected to evaporation with gold (metallization) in sputter and were observed by a SEM. **The osteoblastic counting cells in all groups were carried out in periods of 24 hours, 72 hours and 7 days, to check the viability and cell growth. **The macrostructural analysis of the PLGA BMM scaffolds presented pores with a diameter varying from 150 to 410μ+m. It was also observed the interconnection of the pores with the dense part well defined and coherent. It was also possible to observe micropores, varying between 10 and 80μ+m.**Scaffold cell interaction demonstrated that the cell culture methodology was positive presenting scattering, adsorption and cell differentiation results, both in the control groups and in the inoculated biomaterial cell interaction samples. The observation of these conditions was carried out by increasing (50X, 100X and 500X) under SEM. Cells were observed highlighting the process of cytoplasmic retraction, probably beginning the process of cell division, which, in addition to the fact of biocompatibility, refers to the cell cell interaction.**The cells on the PLGA BMM scaffolds presented normal morphology in relation to the control group, indicating biocompatibility of the material. The cells were well adhered, grown and well spread on the scaffolds, denoting adequate cell material interaction.**
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