Online Library
Argon Plasma treatment affects early biological responses to graft material surfaces- an in vitro comparative study.
EAO Online Library. Federico M. Oct 9, 2018; 232586; P-BR-4
Mussano Federico
Mussano Federico
Login now to access Regular content available to all registered users.
Discussion Forum (0)
Rate & Comment (0)
Enhancement of the bio-reactivity of graft materials to bone forming cells and proteins is the ideal aim in the regenerative material research field.**From a physic-chemical standpoint, Argon plasma treatment (PAT) and ultraviolet irradiation (UV) were demonstrated to increase the titanium surface energy.This in vitro study aimed to test the effect of a PAT and UV on graft materials assessing surface topography, osteoblasts growth and cell viability.****Synthetic hydroxyapatite (Mg-HA), Biphasic calcium phosphate (BCP), cancellous and cortical animal bone matrices (CaBM, CoBM) were used as graft samples representing commonly used classes of graft materials. Sixty-six serially numbered disks of 10mm in diameter were used for each graft material and randomly divided into three groups- test 1 (PAT), test 2 (UV) and control (absence of treatment) groups.**Six samples underwent topographic analysis using SEM pre- and post-treatments to evaluate any eventual topographic difference. Additionally, cell adhesion (using two human osteoblast models (MG63, Saos-2) in a bio-reactor was analyzed on test and control samples. Cell proliferation was analyzed after 72h.**Finally, inflammatory reaction of any treatment was analyzed using human macrophages viability (cell line U-937). Due to the nonparametric nature of the data collected, differences between groups were analyzed using the Mann–Whitney test (p value<0.05)Topographic analysis showed that, due to the manufacturing modalities, Mg-HA and BCP disks have a flat morphology, while BM samples are extremely porous and characterized by high roughness. Post-treatment analysis failed to highlight any significant visible difference. **At the early stage, Plasma treatment significantly increased the cell adhesion on all different grafts while UV failed to show any significant increase (Fig 1).**After 72h, this phenomenon disappeared due to the saturation effect (Fig 2).**Viability test showed no differences in terms of macrophage adhesion (Fig 3).Within the limitations of this study, data reported confirmed that non-atmospheric plasma of Argon treatment increased cell adhesion on all classes of graft materials in a similar extent without any negative inflammatory effect. **These results might clinically imply the use of Plasma of argon to enhance the reactivity of graft materials when used in guided bone regenerative procedures.
Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.

Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.

Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.

Save Settings