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Resins for stereolithographic 3D printing- The impact on L929 cells and human oral fibroblasts
EAO Online Library. AGIS H. Oct 9, 2018; 232552; P-BR-148
Hermann AGIS
Hermann AGIS
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Additive manufacturing is used for the production of surgical guides and splints, retainers, aligners. Desktop stereolithography (SLA) 3D printers and the respective resins have been developed for effective production of these devices in dental laboratory and dental offices. However, the 3D printing of the devices still requires several processing steps.To reveal how the processing changes the properties of the different resins was the aim of the study. We evaluated the impact of such resins on cells of soft tissue.Resins at different stages of processing were tested for their impact on the activity of L929 cells and human oral fibroblasts in monolayer culture models and spheroid culture models. MTT, resazurin-based toxicity assays and live-dead staining was applied.We found that the effect of the resins on L929 and oral fibroblasts depends on the stage of manufacturing in which they were assessed. Liquid resins induced a stronger reduction of cell activity. Resin specimens after printing and in particular after post curing exhibited a weaker the impact on cell activity and cell death.We conclude that the processing including post curing of the resins has a major impact on the response of L929 cells and oral fibroblasts. Our data support the notion that adherence to processing guidelines is of high relevance for the production of devices for application in dentistry.
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