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Study on the relationship between microbial composition around implants and periodontal status
EAO Online Library. Zhou N. Oct 9, 2018; 232473; P- PB- 21
Na Zhou
Na Zhou
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Biofilm ecosystem around the surface of teeth or prostheses is composed of more than 700 bacteria species, which are closely related to periodontal and peri-implant diseases. Though periodontal pathogens can be detected around the implants based on the theory of bacteria translocation, the microbiota is usually not identical between peri-implant and periodontal sites. Moreover, it’s unclear whether there are differences of bacteira composition in peri-implant sites in diverse periodontal status.To investigate the bacterial diversities in peri-implant biofilms and the differences in bacteria composition between periodontitis and periodontal healthy patients by 16S rRNA sequencing technique.Patients with or without periodontitis completed implant restoration at the first molar site for at least six months were investigated. Subgingival plaques were collected around the involved implants and the first molars. Then periodontal examination was provided, but the recorded probing depth were limited to the sampling sites. Plaque samples were transported to the laboratory and the bacterial DNA was extracted. After the V4 region of the 16S rDNA was amplified, the mixture PCR products were purified, processed and sequenced on Illumina HiSeq platform. The effective data were clustered as OTUs (Operational Taxonomic Units) and the prevalence and identity of bacteria was analyzed using Sliva database and advanced techniques.36 implants in 30 periodontitis subjects (PI) and 13 implants in 11 healthy subjects (HI) were included in our study. Peri-implant probing depth was comparable between two groups (P>0.05). The average OTUs was 551, corresponding to 2 kingdoms, 50 phyla, 127 classes, 185 orders, 362 families, 783 generas. The genera Filifactor, Fusobacterium, Porphyromonas, Neisseria, Fretibacterium were present in higher abundance in PI, while Corynebacterium, Actinomyces, Streptococcus, Gemella and Veillonella were higher in HI. Anosim analysis indicated bacterial diversity between PI and HI was significantly different (P<0.05). T-test showed phylum Actinobacteria was higher in HI (P<0.05). Phylum Bacteroidetes, Spirochaetes, TM7 (Saccharibacteria), Synergistetes, SR1 (Absconditabacteria) and Tenericutes were higher in PI (P<0.05). LEfSe analysis were carried out and find genera Actinomyces and Sterptococcus in HI, Porphyromonas and Treponema 2 in PI could be considered as biomarkers individually.**The biofilm around implant was comparable between periodontitis and healthy subjects, and it was mainly consisted of Gram-negative anaerobic bacteria. The abundance of disease-associated generas, such as Porphyromonas, Treponema, was higher in PI while genera Actinomyces and Streptococcus were higher in HI. Periodontitis history is considered as a risk factor for implant failure, and our study illustrates that more harmful bacteria colonizes in peri-implant sulcus in patients with periodontitis.
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